An ultrathin water layer separates the membrane from the solid surface by a distance of 10-20 A. Solid-supported membranes or solid-supported lipid bilayers (s-SLBs) consist of a continuous lipid bilayer deposited onto a planar solid substrate. For example, they allow the investigation of lipid-lipid and cell-cell interactions, cell fusion, the functional role of membrane proteins, membrane-protein interactions, as well as other biochemical processes such as molecular transport, signaling, and catalysis. These two-dimensional soft systems are choice models to study the structure and function of the cellular membrane and its components. , supported lipid bilayers (SLBs) are commonly used as versatile biological membrane mimics. Keywords: supported membrane phospholipid bilayer polymer cushion polydopamine lipid diffusionįirst reported in the early 1980s by McConnell et al. Diffusion coefficients of ~5.9 and 7.2 |im2 s-1 were respectively determined for DMPC and DOPC at room temperature, values which are characteristic of lipids in a free standing bilayer system. Polydopamine films preserved the lateral mobility of the phospholipids as shown by fluorescence microscopy recovery after photobleaching (FRAP) experiments. A zwitterionic phospholipid bilayer was then deposited on the polydopamine cushion by fusion of dimyristoylphosphatidylcholine (DMPC) and dioleoylphosphatidylcholine (DOPC) vesicles. Atomic force microscopy imaging indicated that these films were also soft with a surface roughness of 2 nm under hydrated conditions. First, 20 nm thick polydopamine films were formed on mica substrates. Received: 8 October 2012 in revisedform: 27November 2012 /Accepted: 29 November 2012 / Published: 4 December 2012Ībstract: We report the formation of lipid membranes supported by a soft polymeric cushion of polydopamine. 6128, Succursale Centre-Ville, Montréal, Québec H3C 3J7, Canada E-Mail: Authors to whom correspondence should be addressed E-Mails: (J.P.C.) (I.M.) Tel.: +1-51 (ext.
6128, Succursale Centre-Ville, Montréal, Québec H3C 3J7, Canada E-Mail: of Pharmacy, Université de Montréal, C.P. 8888, Succursale Centre-Ville, Montréal, Québec H3C 3P8, Canada E-Mail: of Chemistry, Université de Montréal, C.P. Claverie '* and Isabelle Marcotteġĝepartment of Chemistry, Université du Québec à Montréal, C.P. Souryvanh Nirasay, Antonella Badia, Grégoire Leclair, Jerome P.
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